Translational_Unit

Part:BBa_K917006:Design

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-08-10)


E. coli cytochrome maturation gene cluster ccmA-H


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 629
    Illegal BamHI site found at 3418
    Illegal BamHI site found at 3646
    Illegal BamHI site found at 5990
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 283
    Illegal AgeI site found at 1110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 4697


Design Notes

Obtained by PCR amplification of E coli JM109 gene cluster


Source

Escherichia coli JM109 chromosomal DNA

References